The Harvest Time, Optimal Growth and Catharanthine Content of Catharanthus roseus Cell Aggregates Treated with Tryptophan in Erlenmeyer Flash

10 pages•Published: March 1, 2021

Dingse Pandiangan, Wenny Tilaar and Nelson Nainggolan

Abstract

Catharanthine bioactive compounds from Catharanthus roseus L. (G.) Don has been studied either in vitro and in vivo. Studies have been conducted to determine the effect of precursor tryptophan on the optimal growth and catharanthine content produced by cell aggregate cultures of Catharanthus roseus. The optimal growth and catharanthine content occur when the aggregate cell growth is good and the content of catharanthine is also high. Aggregate cell C. roseus was cultured on Murashige and Skoog (MS) with the addition of 2 mg/L NAA and 0.2 mg/L kinetin. Treatment was given with the addition of 150 mg/L tryptophan and the control without tryptophan. Further, the growth and the content of catharanthine were observed in cell aggregates and the medium on day 0, 4, 7, 10, 14, and day- to-21. Catharanthine content analysis was conducted using High-Performance Liquid Chromatography (HPLC) using a VP-ODS column C-18. The optimal growth was different in control and that 150 mg/L treatment tryptophan of cell aggregates and tryptophan may improve the growth and lead to longer growing phase after treatment compared with controls. The growth and catharanthine content are the highest after the tryptophan treatment on cell aggregates occurred on day 14 with the content of 2261.68 ± 17.05 mg/g DW. Subculture and harvest time are the best cells on day 14 after culture, and on day 14 the highest catharanthine content and biomass were obtained. The results of this study are needed to determine the appropriate harvest time on the production of secondary metabolites of catharanthine by in vitro. For future research, the optimal growth and content of catharanthine on the 14th day of culture in the bioreactor in a large scale or industries should be observed.

Keyphrases: catharanthine content, cell aggregate, harvest time, optimal growth, tryptophan treatment

In: Kustiati and Wolly Candramila (editors). Proceedings of KOBI 2nd International Conference on Management of Tropical Biodiversity for Human Welfare: From Ecosystem to Molecular, vol 1, pages 96-105.

Links:	https://easychair.org/publications/paper/p7CK
	https://doi.org/10.29007/9mjb

BibTeX entry

@inproceedings{KOBI-2019:Harvest_Time_Optimal_Growth,
  author    = {Dingse Pandiangan and Wenny Tilaar and Nelson Nainggolan},
  title     = {The Harvest Time, Optimal Growth and Catharanthine Content of Catharanthus roseus Cell Aggregates Treated with Tryptophan in Erlenmeyer Flash},
  booktitle = {Proceedings of KOBI 2nd International Conference on Management of Tropical Biodiversity for Human Welfare: From Ecosystem to Molecular},
  editor    = {Kustiati and Wolly Candramila},
  series    = {EPiC Series in Biological Sciences},
  volume    = {1},
  publisher = {EasyChair},
  bibsource = {EasyChair, https://easychair.org},
  issn      = {2398-5291},
  url       = {/publications/paper/p7CK},
  doi       = {10.29007/9mjb},
  pages     = {96-105},
  year      = {2021}}

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