Antibacterial and Antioxidant Properties of Murraya Paniculata Leaves via Methanol Extraction

EasyChair Preprint 11150

Abstract

Murraya paniculata (M. paniculata), a well-known medicinal plant, has widely been used in Ayurveda and other oriental healing practices for the treatment of a number of human diseases. The extract of M. paniculata can be prepared from different parts of the plant, especially the leaves. The main objective of this study is to evaluate the antibacterial and antioxidant activities of the methanolic extract of M. paniculata leaves using DPPH and disk diffusion methods. M. paniculate methanolic extract (MpME) was prepared from the leaves using the maceration method, which resulted in an extract yield of 13.27%. The 1,1-diphenyl-2-picrylhydrazil (DPPH) method was used to evaluate the antioxidant activity of MpME. Ascorbic acid was used as a positive control, and methanol as a negative control to ensure the validity of the assay. The highest radical scavenging activity (RSA) for MpME was 54.47% ± 0.51 at a concentration of 1000 μg/mL (IC₅₀ value: 1862 μg/mL). The antibacterial activity of MpME was evaluated by the agar disk diffusion method against two bacterial strains: a Gram-positive bacterium (Staphylococcus aureus (ATCC 12600) and a Gram-negative (Pseudomonas aeruginosa (ATCC 10145). Each assay was done in triplicate. In this assay, the positive controls vancomycin and gentamicin were used as indicators to prove the validity of the assay. The Gram-positive bacteria were more susceptible to the extract (average diameter of inhibition zone for Staphylococcus aureus = 6 ± 0.06 mm) than the Gram-negative bacteria (average diameter of inhibition zone for Pseudomonas aeruginosa = no inhibition zone). The results of the study show that MpME has antibacterial and antioxidant properties. Thus, these findings indicated that MpME could be developed as an antioxidant and antibacterial agent in various applications.

Keyphrases: Murraya paniculata extract, Phenol, Phytochemical, antibiotic resistance, flavonoid, maceration

@booklet{EasyChair:11150,
  author    = {Mazlin Mohideen and Fatma Hidayah Mohamad and Nur Azzalia Kamaruzaman},
  title     = {Antibacterial and Antioxidant Properties of Murraya Paniculata Leaves via Methanol Extraction},
  howpublished = {EasyChair Preprint 11150},
  year      = {EasyChair, 2023}}