Advancing Infectious Disease Research: Development and Characterization of Human Skin and Immune System (hSIS) Humanized Mouse and Rat Models

EasyChair Preprint 12436

Abstract

The human skin, as the primary barrier against pathogen transmission, is a key focus in infectious disease research. Despite the utility of rodent models in studying human-specific skin pathogens, achieving successful co-engraftment of human skin, autologous lymphoid tissues, and immune cells remains a challenge. In this study, we introduce the development of a novel human Skin and Immune System (hSIS)-humanized NOD-scid IL2Rγnull (NSG) mouse and Sprague–Dawley-Rag2tm2hera Il2rγtm1hera (SRG) rat models, involving the co-engraftment of human full-thickness fetal skin, autologous fetal lymphoid tissues, and autologous fetal liver-derived hematopoietic stem cells. The human skin, as the primary barrier against pathogen transmission, is a key focus in infectious disease research. Despite the utility of rodent models in studying human-specific skin pathogens, achieving successful co-engraftment of human skin, autologous lymphoid tissues, and immune cells remains a challenge. In this study, we introduce the development of a novel human Skin and Immune System (hSIS)-humanized NOD-scid IL2Rγnull (NSG) mouse and Sprague–Dawley-Rag2tm2hera Il2rγtm1hera (SRG) rat models, involving the co-engraftment of human full-thickness fetal skin, autologous fetal lymphoid tissues, and autologous fetal liver-derived hematopoietic stem cells.

Keyphrases: Human skin, Humanized Mouse, infectious disease

@booklet{EasyChair:12436,
  author    = {Liis Tammik},
  title     = {Advancing Infectious Disease Research: Development and Characterization of Human Skin and Immune System (hSIS) Humanized Mouse and Rat Models},
  howpublished = {EasyChair Preprint 12436},
  year      = {EasyChair, 2024}}