Development of a Nanofiber Wound Dressing Prototype and Evaluation of in Vitro Biocompatibility

EasyChair Preprint 7725

Abstract

In this study, it was aimed to develop a two-layer wound dressing prototype containing Polycaprolactone (PCL)/Collagen (COL) and PCL/St. St. John's Wort oil/Momordica charantia maceration oil, which will accelerate wound healing, minimize infection and provide a suitable moist environment. The evaluation of in-vitro biocompatibility of dressing was also aimed.

Scanning electron microscope (SEM) analyzes showed that PCL/COL containing fibers had a diameter of about 135 nm, while the diameter of nanofibers containing PCL/St. John's Wort oil/Momordica charantia maceration oil was approximately 513 nm. According to thermal and gravimetric analysis (TGA) results, thermal degradation temperature ranges of PCL/St. John's Wort oil/Momordica charantia maceration oil, PCL/COL and double layer nanofibers were found 275-410 °C, 300-430 °C and 350-440 °C, respectively. Weight losses due to temperature increase were found to be approximately 88.4%, 84.32% and 88.1% for PCL/St. John's Wort oil/ Momordica charantia maceration oil, PCL/COL and double layer nanofibers, respectively. As an in vitro study, L929 normal fibroblast cells were grown with the wound dressing prototype for 24 hours, 72 hours, and 144 hours. Normal fibroblast cell proliferation was found to be 60.4%, 80.18% and 66.71% after 24, 72, and 144 hours incubation period, respectively.

Keyphrases: Electrospinning, Electrospun nanofiber, Hypericum perforatum, Momordica charantia, Wound Dress

@booklet{EasyChair:7725,
  author    = {Emre Fatih Edİz and Cansu Güneş Çİmen and Deniz Ümmetoğlu and Meltem Demİrel Kars and Ahmet Avci},
  title     = {Development of a Nanofiber Wound Dressing Prototype and Evaluation of in Vitro Biocompatibility},
  howpublished = {EasyChair Preprint 7725},
  year      = {EasyChair, 2022}}